Intermediate

Part:BBa_K316014:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-23)


Dif sequence followed by PmeI recognition site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be cloned using standard biobrick methods. Two single stranded, synthetic oligos were annealed to produce a double stranded DNA sequence with single stranded overhangs identical to the product of digestion by EcoRI and SpeI. Thus compatible with biobrick cloning methods.


Source

Oligonucleotide synthesis of single stranded primers.

References